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Role of Pselectin and antiPselectin monoclonal antibody in apoptosis during liver and renal ischemiareperfusion injury
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- Author:
- No author available
- Journal Title:
- CHINESE CRITICAL CARE MEDICINE
- Issue:
- 4
- DOI:
- 10.3760/j.issn:1003-0603.1999.04.007
- Key Word:
- 缺血再灌注损伤;细胞凋亡;P选择素;单克隆抗体,P选择素
Abstract： 目的:探讨P选择素及其单克隆抗体在肝、肾缺血再灌注时其表达对细胞凋亡的影响.方法:建立大鼠肝及肾缺血再灌注损伤模型,分别采用免疫组化链菌素抗生物素蛋白生物素酶标法和原位DNA片断末端标记法检测有或无P选择素单抗治疗组肝、肾组织中P选择素表达及凋亡细胞.结果:大鼠肝、肾缺血再灌注时P选择素在肝、肾内广泛表达,肝、肾组织出现显著病理改变并发生细胞凋亡;P选择素单抗作用组肝、肾组织中P选择素未表达,无明显病理变化且细胞凋亡减少.结论:在肝、肾缺血再灌注时,P选择素介导了肝、肾内中性粒细胞积聚和细胞凋亡,并加重肝、肾损伤;抑制P选择素可减少肝、肾内中性粒细胞积聚和细胞凋亡,减轻肝、肾缺血再灌注损伤.
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