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Effects of antiendotoxin agents on bacterial translocation and tumor necrosis factorα mRNA expression in rats after thermal injury
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- Author:
- No author available
- Journal Title:
- CHINESE CRITICAL CARE MEDICINE
- Issue:
- 1
- DOI:
- 10.3760/j.issn:1003-0603.1999.01.003
- Key Word:
- 灼伤;多粘菌素B;杀菌/通透性增加蛋白;细菌易位;肿瘤坏死因子α
Abstract： 目的:比较两种不同内毒素拮抗剂--多粘菌素B和杀菌/通透性增加蛋白(BPI)对大鼠烫伤后肠道细菌易位和组织肿瘤坏死因子α(TNFα)基因表达的影响.方法:采用大鼠35%体表面积Ⅲ度烫伤模型,动物随机分为正常对照组(8只)、烫伤组(24只)、多粘菌素B治疗组(7只)和BPI治疗组(6只).结果:大鼠烫伤后肠道细菌易位率明显上升,伤后8小时细菌易位率达37.1%,与正常对照组比较差异显著(P<0.01);细菌易位率最高的器官是肠系膜淋巴结,不同器官易位细菌量无显著性差异;给予烫伤大鼠多粘菌素B和BPI治疗均可不同程度地降低细菌易位率,其细菌易位率分别降至烫伤8小时组的61.7%和53.9%;BPI比多粘菌素B更为有效地降低肠、肾等组织TNFα mRNA表达水平(P均<0.01).结论:多粘菌素B和BPI均可降低大鼠烫伤后肠道细菌易位的发生;BPI较多粘菌素B能更有效地抑制多种组织TNFα mRNA表达.因此,烧伤早期应用BPI治疗对抑制创伤后机体过度炎症反应具有重要意义.
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