Expression of Na+-H+ exchanger 1 in human gastric carcinoma tissue and its clinical significance
- Author:
- No author available
- Journal Title:
- CHINESE JOURNAL OF GASTROINTESTINAL SURGERY
- Issue:
- 8
- DOI:
- 10.3760/cma.j.issn.1671-0274.2010.08.018
- Key Word:
- 胃肿瘤;Na+-H+交换泵;聚合酶链反应;蛋白质印迹法;Stomach neoplasms;Na+-H+ exchanger;Polymerase chain reaction;Western blotting
Abstract： Objective To determine the expression of Na+-H+ exchanger 1(NHE1)in human gastric carcinoma tissue and to investigate the association between NHE1 expression and clinicopathological characteristics.Methods The expressions of NHE1 mRNA and protein were detected in both gastric carcinoma tissue(n=60)and adjacent gastric mucosa tissue (n=30)by reverse transcription polymerase chain reaction(RT-PCR)and Western blot.The association between the expression and the clinicopathological characteristics was analyzed.Results The relative expression levels of NHE1 mRNA and protein in gastric carcinoma tissue were 0.786±0.291 and 1.442±0.175.which were significantly higher than those in adjacent gastric mucosa tissue(0.369±0.052 and 0.348±0.029)(P<0.01).The expression of NHE1 mRNA was positively correlated with NHE 1 protein in the gastric carcinoma tissue (r=0.264,P<0.05).The expressions of NHE1 mRNA and protein were associated with the depth of invasion,lymph node metastasis,and TNM staging(P<0.05).However,no statistical difference was found in age,gender,and tumor differentiation(P>0.05).Conclusion The expression levels of NHE1 mRNA and protein are significantly up-regulated in gastric carcinoma tissue,which may be involved in the development of gastric carcinoma.
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