Abstract： Objective To investigate the effects of duodental-jejunal bypasss (DJB) and sleeve gastrectomy (SG) on the expression of liver glucokinase (GCK) in diabetic rats. Methods Animal models of Goto-Kakizaki rats and Sprague-Dawley rats were established by DJB and SG. Results of fasting glycemia and insulin were compared. Liver tissue was harvested 8 weeks postoperatively. Quantitative real-time PCR and Western blot were used to detect liver GCK mRNA and protein expression after operation. Results Fasting plasma glucose levels of DJB group and SG group in GK rats were markedly declined 3 day and 1, 2, 4, 6, 8 weeks postoperatively (all P<0.01), while Sham group only droped 3 day and 1 week postoperatively, and there were no significant differences 2 weeks postoperatively (P>0.05). Fasting plasma glucose levels of each group in SD rats did not ehange after operation. In GK rats, GCK mRNA level (1.45±0.29) and protein expression (494.25±30.25) after DJB were higher than Sham group (1.05±0.19 and 409.13±26.86) and control group (1.04±0.17 and 404.75± 30.90). GCK mRNA level and protein expression after SG were 0.65±0.25 and 345.25±28.13 respectively, which were significantly lower than those in control group (all P<0.01). All the groups in SD rats experienced similar GCK expression change. Conclusion Both DJB and SG can decrease the plasma glucose levels of GK rats, while there are different effects on the expression of liver GCK.