Abstract： Objective To explore the significance of clinical application of carbon nanoparticles labeled lymph node in curative resection for colorectal carcinoma.Methods With perspective cotrolled randomly research， 60 patients with colorectal carcinoma were randomly divided into two groups：carbon nanopartieles was injected into the periphery of the tumor in 30 patients before or during the operation，30 patients were as control without any injection during operation.The number of lymph nodes(dyed or not dyed)and the location of metastasic nodes were recorded separately.Results (1)In the labelling group，the average number of eliminated lymph nodes(12.6，6～18)was significantly more than that of the eontrbl group(8.1，4～14)(P=0.0065).The ratio of dyed lymph nodes was 51.9％.(2)The check-up ratio with histology of metastasic lymph nodes in labelling group was higher than that in the control group.The metastasis rate of dyed lymph nodes 33.6％was more sensitive than the control group 24.7％(P=0.014)，especially for＜4 mm nodes(P=0.0021).(3)In two cases during operation,two dyed lymph nodes were found at the root of inferior mesenteric artery and along the side of internal iliac artery， so we the dissection with positive diagnosis was extended. Conclusion The technique of carbon nanopartieles labeled lymph nodes is easy and effective.The dyed lymph nodes can be easily identified and can be used as a guide for lymph nodes dissection in colorectal carcinoma operation.