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Detection of microsatellite instability in colorectal cancer by fluorescence multiplex polymerase chain reaction and its clinical value
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- Author:
- No author available
- Journal Title:
- CHINESE JOURNAL OF GASTROINTESTINAL SURGERY
- Issue:
- 3
- DOI:
- 10.3760/cma.j.issn.1671-0274.2007.03.006
- Key Word:
- 结直肠肿瘤;聚合酶链反应,多重荧光;微卫星不稳定
Abstract： 目的 探讨多重荧光PCR方法检测结直肠癌微卫星不稳定(MSI)及其临床意义.方法 将2004-2005年间进行手术治疗的110例结直肠癌患者建立队列,以多重荧光PCR方法检测结直肠癌MSI,并对MSI和微卫星稳定(MSS)结直肠癌患者的临床病理特点进行比较.结果 多重荧光PCR方法扩增出所有患者的5个微卫星序列.其中MSI-H 10例(8.1%),MSI-L 13例(11.8%),MSS为87例(79.1%).共检测BAT-26变异9例(8.2%)、BAT-25变异11例(10.0%)、D2S123变异11例(10.0%)、D5S346变异6例(5.5%)和D17S250变异8例(7.3%).MSI-L、MSI-H和MSS组结直肠癌患者年龄比较,差异有统计学意义(P＜0.05);其他临床病理特点差异无统计学意义(P＞0.05).结论 多重荧光PCR方法检测MSI结果稳定,宜于临床应用;MSI和MSS结直肠癌患者临床病理特点比较未见差异.
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