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The role of AMPK gene in glucose and lipid metabolism in C57BL/6J mice
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- Author:
- No author available
- Journal Title:
- Chinese Journal of Diabetes
- Issue:
- 12
- DOI:
- 10.3969/j.issn.1006-6187.2011.12.015
- Key Word:
- 糖尿病;腺苷酸活化蛋白激酶;C57BL/6J小鼠
Abstract： 目的 研究腺苷酸活化蛋白激酶( AMPK)基因在C57BL/6J小鼠糖脂代谢中的作用.方法 分别取5周龄AMPK基因敲除(AMPK-KO)小鼠和C57BL/6J对照小鼠各24只,分为正常饲料(ND)喂养和高脂高糖饲料(HFD)喂养两组.喂养12周,每两周测定小鼠禁食4h血糖(FBG),实验结束前行口服糖耐量实验(OGTT),解剖取样,检测血生化指标、脂酶活性及相关蛋白的表达. 结果 AMPK-KO小鼠血糖、TC、LDL-C、HbA1 c、6-磷酸葡萄糖脱氢酶(G6PD)活性、糖原合成酶激酶(GSK)活性、肝脏PPARγ蛋白表达量明显高于对照小鼠(P＜0.05)；其胰岛素含量、肝糖原含量、肌糖原含量、肝脂酶(HL)活性、脂蛋白酯酶(LPL)活性、总脂酶活性、葡萄糖激酶(GK)活性、肝组织P-AMPK蛋白、葡萄糖转运蛋白4(GluT-4)蛋白的表达量低于对照组(P＜0.05). 结论 AMPK基因通过调节C57BL/6J小鼠糖脂代谢,在T2DM的发病中起重要作用.
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