The serum level of advanced oxidation protein products(AOPP) and its role in the genesis and development of diabetic nephropathy
- Author:
- No author available
- Journal Title:
- Chinese Journal of Diabetes
- Issue:
- 10
- DOI:
- 10.3969/j.issn.1006-6187.2011.10.005
- Key Word:
- 糖尿病肾病;晚期蛋白氧化产物;超氧化物歧化酶;单核细胞趋化蛋白-1
Abstract： 目的 通过检测不同时期糖尿病肾病(DN)患者血清晚期蛋白氧化产物(AOPP)水平,分析其与超氧化物歧化酶( SOD)及单核细胞趋化蛋白-1(MCP-1)的相关性,研究蛋白氧化与DN的关系及临床意义.方法66例2型糖尿病(T2DM)患者根据尿白蛋白排泄率(UAER)将患者分为以下3组:正常白蛋白尿(N-UAlb)组20例(UAER＜30 mg/24h)、微量白蛋白尿(M-UAlb)组24例(UAER 30～300mg/24h)和大量白蛋白尿(L-UAlb)组22例(UAER＞300 mg/24h),并与20名正常对照(NC)组比较.用Wikto-sarsat介绍的方法改进后测定各组的血清AOPP水平,黄嘌呤氧化酶法测定SOD水平,ELISA法测定血清MCP-1水平.结果随着UAER的增加,血清中AOPP水平逐渐增高,各组间比较均有统计学意义(P＜0.05)；血清中SOD水平逐渐降低,除N-UAlb组与NC组间相比外,其余各组间比较均有统计学意义(P＜0.05)；血清MCP-1水平逐渐增加,除L-UAlb组与M-UAlb组间相比外,其余各组间比较均有统计学意义(P＜0.05).相关分析表明血清.AOPP与SOD呈显著负相关,与BUN、Cr、TG、UAER呈显著正相关,与MCP-1、HDL-C、LDL-C、TC、HbA1 c、BMI无相关性.血清SOD与BUN、Cr、UAER、MCP-1呈显著负相关,与TG、TC、LDL-C、HDL-C、HbA1 c、BMI无相关性.多重线性同归分析结果显示Cr、TG、UAER对AOPP有显著影响；Cr、UAER、MCP-1对SOD有显著影响.结论增加的蛋白氧化损伤及氧化应激状态可能在DN的发生发展中起重要作用,增加的蛋白氧化应激可能与DN的氧化应激状态和慢性炎症相关.
- [1]B, Descamps-Latscha,V, Witko-Sarsat.Importance of oxidatively modified proteins in chronic renal failure.[J].2001,78.
- [2]Tohru, Fukai,Rodney J, Folz,Ulf, Landmesser,etc.Extracellular superoxide dismutase and cardiovascular disease.[J].2002,55(2).
- [3]V, Witko-Sarsat,M, Friedlander,C, Capeillère-Blandin,etc.Advanced oxidation protein products as a novel marker of oxidative stress in uremia.[J].1996,49.
- [4]Fujita H,Fujishima H,Chida S,etc.Reduction of renal superoxide dismutase in progressive diabetic nephropathy.[J].2009,20(6).
- [5]Warwas M,Piwowar A,Knapik-Kordecka M,etc.Plasma glycooxidation protein products in type 2 diabetic patients with nephropathy.[J].2008,24(7).