Abstract： Objective To observe the clinical effectivity and safety of Glargine treatment in perioperative patient with type 2 diabetes. Methods 60 T2DM patients scheduled for surgery were divided into glargine insulin group (Gla, n=30) and MDI group (Humulin N, n=30). The Dosage was adjusted according to a blood glucose fluctuations to compare the effect and safety between the two groups. Results The levels of blood glucose in the two groups were reduced after treatments (P<0.01) and there is no significant difference in hypoglycemic effect between the two groups (P>0.05). But the blood glucose fluctuation was smaller in Gla group than in MDI group and the glucose target arrival time was shorter in Gla group than in MDI group (P<0.01). The insulin dosage, the incidences of hypoglycemia and wound infection and delayed healing rate were less in Gla group than in MDI group (P<0.05). Conclusions Glargine treatment can effectively, rapidly and savely control the blood glucose and is especially suitable for the perioperative patient requiring a sufficient basal insulin.