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Effects of extracts of Lonicera japonica Thanb.on hepatic PGC-1α expression and insulin resistance in mouse

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Author:
No author available
Journal Title:
CHINESE JOURNAL OF DIABETES
Issue:
3
DOI:
10.3969/j.issn.1006-6187.2011.03.0011
Key Word:
过氧化物酶体增殖物激活受体γ共激活因子-1α;胰岛素抵抗;金银花;ob/ob小鼠;Peroxisome proliferators-activated receptor-γ coactivator-1;Insulin resistance; Lonicera japonica thunb; ob/ob mice

Abstract: Objective To explore the effects of extracts of Lonicera japonica Thunb. on hepatic PGC-1α expression and insulin resistance in mice and L02 cell. Methods C57/B6J mice were divided into control group (group A)and Lonicera japonica Thunb.-treated group (group B). The ob/ob mice were divided into control group (group C)and Lonicera japonica Thunb.-treated group (group D). L02 cells cultured in normal medium were divided into control group (group a) and Lonicera japonica Thunb.-treated group(group b). L02 cells cultured in medium with FFA (free fatty acid) were divided into control group(group c)and Lonicera japonica Thunb.-treated group (group d). The basal blood glucose and insulin were measured and insulin tolerance test(ITT) was done in ob/ob mice.PGC-1α mRNA and protein expressions were detected by q-RT-PCR and Western blot respectively. Results Hepatic mRNA expression of PGC-1α were significantly decreased in group d versus group c(P<0.05) and in group D versus group C(P<0.05) , while were similar in group b versus group a and in group B versus group A (all P>0.05).Hepatic protein expression of PGC-1α was significantly lower in group D than in group C(P<0.01). Basal blood glucose were significantly lower in group D than in group C (P<0.05), while insulin secretion were similar (P>0.05). Hepatic insulin resistance in group D was improved according to the results of ITT. Conclusions Lonicera japonica Thunb. decreases the pathologically high expression of PGC-1α mRNA in liver and basal blood glucose, and improves insulin resistance .

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