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Effect of exercise on phosphorylation of serine307/tyrosine in insulin-resistant rats
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- Author:
- No author available
- Journal Title:
- CHINESE JOURNAL OF DIABETES
- Issue:
- 5
- DOI:
- 10.3969/j.issn.1006-6187.2010.05.004
- Key Word:
- 运动;胰岛素抵抗;大鼠;丝氨酸磷酸化;酪氨酸磷酸化
Abstract： 目的 观察运动对高脂膳食诱导胰岛素抵抗(IR)大鼠骨骼肌胰岛素受体底物1(IRS-1)丝氨酸307(Ser307)磷酸化和酪氨酸(Tyr)磷酸化通路的影响.方法 Wistar大鼠75只,分为正常对照(NC)组、高脂膳食(HF)组.10周后再将HF组分为高脂膳食运动(HFE)组和高脂膳食非运动(HFNE)组.以钳夹技术观察各组大鼠胰岛素敏感性(IS)变化,Western Blot法检测大鼠骨骼肌IRS-1 Ser307磷酸化和Tyr磷酸化水平.结果 (1) 10周后HF组葡萄糖输注率(GIR60～120)明显低于NC组;与NC组比较,HF组IRS-1 Tyr磷酸化水平降低,IRS-1 Ser307磷酸化水平升高(P均＜0.01).(2) 14周后HFE组GIR60～120明显高于HFNE组;与HFNE组比较,HFE组IRS-1 Tyr磷酸化水平升高,IRS-1 Ser307磷酸化水平降低(P均＜0.01).结论 运动干预改善IR大鼠IS,其机制可能与改善IRS-1 Ser307/Tyr异常磷酸化有关.
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