You Position: Home > Paper

A study on the mechanism of the N-acetylcystein-improved insulin resistance in high-fat-diet rats

( views:260, downloads:89 )
Author:
No author available
Journal Title:
CHINESE JOURNAL OF DIABETES
Issue:
4
DOI:
10.3969/j.issn.1006-6187.2010.04.005
Key Word:
N-乙酰半胱氨酸;胰岛素抵抗;实时荧光定量PCR;N-acetylcystein;Insulin resistance; Real time PCR

Abstract: Objective To study the changes of peripheral insulin resistance in highfatdiet rat models and the effect of Nacetylcystein intervention. Methods59 normal male SD rats aged 8 weeks were randomly divided into 3 groups:normal diet group(NC,n=20), high fat diet group(HF,n=20),and Nacetylcystein treated group (NAC,n=19, Nacetylcystein 300mg?kg-1?d-1 and on high fat diet). At the end of twenty weeks feeding, we determined fasting plasma insulin(Ins),glucose(Glu) and nitrotyrosine, malondialdehyde(MDA), reduced glutathione hormone(GSH). The glucose infusion rate(GIR)was measured by using euglycemic hyperinsulinemia clamp to evaluate the peripheral insulin resistance.The expressions of IRS1,IRS2 gene in muscle were detected by real time PCR. Results (1)The plasma insulin,glucose,nitrotyrosine and MDA concentrations were higher in HF group than in NC group,but GSH level in plasma was lower.NAC intervention could reverse these effects.(2)The GIR was decreased significantly in HF group as compared with NC group(5.25±1.2mg?min-1?kg-1 vs 13.56±1.7mg?min-1?kg-1)(P<0.01), NAC intervention can reverse these effect(9.28±1.5mg?min-1?kg-1vs 5.25±1.2 mg?min-1?kg-1)(P<0.01).(3)The gene expression of IRS1 was decreased by 52.1% in HF group, and the expressions of IRS2 was decreased by 43.5% (all P<0.01).In contrast, the expressions of IRS1,IRS2 in NAC group were reversed by 738% and 68.4% respectively as compared with HF group(P<0.01). ConclusionsAntioxidant therapy could increase the gene expression of insulin signal transduction molecules in muscle, which might relate with the antioxidant effects of NAC.

WanfangData CO.,Ltd All Rights Reserved
About WanfangData | Contact US
Healthcare Department, Fuxing Road NO.15, Haidian District Beijing, 100038 P.R.China
Tel:+86-010-58882616 Fax:+86-010-58882615 Email:yiyao@wanfangdata.com.cn