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The effect of erigeron breviscapus on apoptosis and expression of bax,bcl-2 in kidney of diabetic rats
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- Author:
- No author available
- Journal Title:
- CHINESE JOURNAL OF DIABETES
- Issue:
- 11
- DOI:
- 10.3969/j.issn.1006-6187.2009.11.021
- Key Word:
- 灯盏花素;糖尿病肾病;细胞凋亡;bax/bcl-2
Abstract： 目的 探讨灯盏花素对糖尿病大鼠肾脏细胞凋亡及对bax和bcl-2mRNA表达的影响.方法 健康雄性SD大鼠随机分为正常对照组(NC)、糖尿病组(DM)和灯盏花治疗组(EB),EB组行灯盏花素20mg·kg~(-1)·d~(-1)腹腔内注射.给药后2周和6周处死,检测肾脏细胞凋亡、肾脏bax和bcl-2mRNA的表达.结果 与NC组比较,(1)DM组及EB组肾小管凋亡细胞数明显增多,且DM组高于EB组;(2)DM组肾脏bax、bcl-2mRNA的表达显著增强, bax/bcl-2值明显升高;EB组与DM组比较bcl-2mRNA表达增强,baxmRNA减弱,bax/bcl-2比值降低;(3)EB组及DM组体重下降,肾指数、肾小球面积、体积升高,且EB组较DM组体重增加,肾指数、肾小球面积、体积下降.血尿素氮、肌酐比较:DM组>EB组>NC组.结论 灯盏花素可能通过影响凋亡相关基因bcl-2和bax的表达而抑制肾脏细胞凋亡, 从而发挥肾脏保护作用.
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