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Effects of extracellular matrix glycation on the balance of proliferation and apoptosis in human dermal fibroblasts
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- Author:
- No author available
- Journal Title:
- CHINESE JOURNAL OF DIABETES
- Issue:
- 11
- DOI:
- 10.3969/j.issn.1006-6187.2009.11.018
- Key Word:
- 糖尿病;皮肤;创面愈合;细胞凋亡;细胞增殖;糖基化
Abstract： 目的 探讨糖尿病皮肤细胞外基质(ECM)糖基化改变与细胞增殖凋亡平衡的相关性.方法 DM或非DM患者的皮肤组织和溃疡创面标本组织学观察,同时免疫组化法检测皮肤组织增殖细胞核抗原(PCNA)、凋亡细胞、糖基化终末产物(AGE)和糖基化终末产物受体(RAGE)表达;体外建立糖基化基质,RAGE抗体干预后,检测细胞存活率、细胞周期和凋亡.结果 与对照组相比,DM皮肤胶原萎缩,凋亡细胞比例增高;创面肉芽形成不良,细胞增殖能力减弱,凋亡细胞增加,AGE和RAGE表达增多;体外糖基化基质上接种成纤维细胞存活率明显减少,细胞周期运行障碍,凋亡增加,阻断AGE-RAGE间相互作用可基本缓解糖基化基质对细胞增殖凋亡过程的影响.结论 DM皮肤组织中糖基化ECM蓄积是细胞功能改变的重要环境介质,经RAGE介导影响细胞增殖凋亡平衡,致糖尿病皮肤创伤起点异常,创面难愈.
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