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Protection of rosiglitazone against HIT-T15 cell damage induced by glucosamine
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- Author:
- No author available
- Journal Title:
- CHINESE JOURNAL OF DIABETES
- Issue:
- 8
- DOI:
- 10.3969/j.issn.1006-6187.2009.08.018
- Key Word:
- 葡萄糖胺;罗格列酮;β细胞线粒体;超微结构
Abstract： 目的 观察罗格列酮(RSG)对葡萄糖胺(GLcN)诱导的HIT-T15细胞超微结构损伤的保护作用.方法 以叙利亚仓鼠胰岛β细胞株HIT-T15细胞作为研究对象,分为正常对照组、GLcN+高浓度葡萄糖培养组、GLcN+高浓度葡萄糖+RSG 10-6mol/L培养组和GLcN+高浓度葡萄糖+RSG 10-8mol/L培养组.各组培养48小时.透射电镜下观察HIT-T15细胞线粒体超微结构. 结果 透射电镜下观察HIT-T15细胞,GLcN组可见凋亡细胞及较多的坏死细胞;多数线粒体模糊不清,个别线粒体有肿胀,核周隙变宽内质网空泡变性的病理变化.RSG 10-8mol/L组的HIT-T15细胞超微结构病变明显减轻,与RSG 10-8mol/L组比较,RSG 10-6mol/L组病变无明显改变. 结论 葡萄糖胺可损伤胰岛β细胞株HIT-T15细胞超微结构,罗格列酮可以部分阻止这种损伤.
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