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The changes of glucose-lipid metabolism in ApoE-/- mice with high-fat induced insulin resistance
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- Author:
- No author available
- Journal Title:
- CHINESE JOURNAL OF DIABETES
- Issue:
- 8
- DOI:
- 10.3969/j.issn.1006-6187.2009.08.007
- Key Word:
- ApoE基因敲除小鼠;胰岛素抵抗;糖脂代谢;胰岛素钳夹
Abstract： 目的 建立高脂诱导ApoE基因敲除(ApoE-/-)小鼠胰岛素抵抗(IR)模型,并探讨其糖脂代谢的变化.方法 高脂喂养ApoE-/-小鼠16周建立IR模型,采用3-3H葡萄糖为示踪剂的扩展高胰岛素钳夹技术评价其胰岛素敏感性和糖脂代谢变化. 结果 普通饲料喂养ApoE-/-小鼠(NF组)血浆FFA、TC、TG、LDL-C、HDL-C和Ins明显高于C57BL/6J小鼠对照(NC)组( P<0.01和 P<0.05),而高脂喂养ApoE-/-小鼠(HF组)上述指标又明显高于NF组(P均<0.01),并且FBG也明显高于NF和NC组(P均<0.01).钳夹稳态时,HF组Ins明显高于NF和NC组(P均<0.01),FFA、TC、TG虽被抑制,但仍明显高于NF和NC组(P均<0.01);HF组葡萄糖输注率明显低于NF和NC组(P均<0.01).在钳夹结束时,HF组葡萄糖清除率明显低于NF和NC组(P均<0.01),而肝糖输出率则明显高于NF和NC组(P均<0.01),仅被抑制51%. 结论 ApoE-/- 小鼠存在糖脂代谢紊乱和IR的遗传特征,长期高脂饲养后更为明显.
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