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Alteration of GLUT-4 translocation in OLETF rat cardiomyocytes by incubating the cells with insulin and leptin
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- Author:
- No author available
- Journal Title:
- CHINESE JOURNAL OF DIABETES
- Issue:
- 3
- DOI:
- 10.3969/j.issn.1006-6187.2009.03.020
- Key Word:
- 胰岛素;瘦素;心肌细胞;葡萄糖转运子4
Abstract： 目的 探讨瘦素、胰岛素与OLETF大鼠心肌胰岛素抵抗的关系.方法 培养的12例大鼠心肌细胞分为11组,Western-blot方法检测不同浓度胰岛素、瘦素刺激不同时间葡萄糖转运子4(GLUT-4)转位效应的差异.结果 瘦素和胰岛素促进GluT-4转位作用分别增加了2.28、3.14、3.07、9.06倍.胰岛素促GluT-4转位最佳时间为30min,瘦素最大效应浓度为100nmol/L,最佳刺激时间为30、60min.在OLETF大鼠,联合组GluT-4转位小于单纯胰岛素组,胰岛素组促进GluT-4转位作用也仅为对照组的69%.P均<0.05.结论 胰岛素和瘦素均促进GluT-4转位,呈时间剂量依赖性;OLEFT大鼠心肌细胞存在胰岛素作用障碍,且瘦素与胰岛素联合应用可影响胰岛素的作用.
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