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Effect of obesity on expression of lipid droplets-related protein in skeletal muscle and its significance
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- Author:
- No author available
- Journal Title:
- CHINESE JOURNAL OF DIABETES
- Issue:
- 1
- DOI:
- 10.3969/j.issn.1006-6187.2009.01.019
- Key Word:
- 肥胖;胰岛素抵抗;异位脂质;脂滴相关蛋白
Abstract： 目的 建立肥胖大鼠模型,探讨肥胖对大鼠骨骼肌脂质含量和脂滴相关蛋白表达的影响及其意义. 方法 22只雄性6周龄Wistar大鼠,随机分为两组:对照组(Con组,n=10)喂普通饲料,肥胖组(Ob组,n=12)喂高脂饲料.16周后测量体重、身长,以正葡萄糖-高胰岛素钳夹试验检测胰岛素敏感性;生化方法检测骨骼肌脂质(TG和DAG)含量,Western blot检测骨骼肌组织脂滴相关蛋白的表达水平. 结果 (1)Ob组大鼠体重、身长、Lee指数、附睾及腹膜后脂肪含量均较Con组显著增加.(2)Ob组血浆TG、FFA和FIns水平较Con组为高,TC、FPG与Con组相比无统计学差异,但葡萄糖输注率(GIR)仅为Con组的34%TG.(3)Ob组大鼠骨骼肌TG、DAG磷酸化周脂素和小凹蛋白3分别较Con组增加184%、35%、42%和26%. 结论胰岛素抵抗肥胖大鼠存在明显的骨骼肌脂质异常沉积,并且其骨骼肌脂滴相关蛋白表达上调.
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