Abstract： Objective To investigate the application of insulin sensitivity index and insulin secretion function index calculated by the new homeostasis model in clinic.Methods Eighty Chinese volunteers in Tianjin area (31 subjects for NGT group, 26 subjects for IGR group and 23 subjects for DM group) were involved in this study. Fasting blood samples were collected to measure the values of plasma glucose, insulin, true insulin and C peptide. Three insulin sensitivity indices (HOMA2-%S-Ins, HOMA2-%S-TI and HOMA2-%S-C) and three insulin secretion function indices (HOMA2-%B-Ins, HOMA2-%B-TI and HOMA2-%B-C) were calculated by the HOMA2 calculator. Results Among the three insulin sensitivity indices, the HOMA2-%S-TI calculated from plasma glucose and true insulin was relatively best (P<0.01) in distinguishing the insulin sensitivity of three groups, the HOMA2-%S-Ins calculated from plasma glucose and insulin was relatively better (0.05>P>0.01) and the HOMA2-%S-C calculated from plasma glucose and C peptide was relatively worse (P>0.05). After adjusted by HOMA2-%S-TI, the abilities of three insulin secretion function indices in distinguishing the insulin secretion function among three groups were very similar (F=60.3, 58.2 and 58.2 respectively, P<0.01), the abilities of them in distinguishing the insulin secretion function between IGR group and DM group were similar, meanwhile HOMA2-%B-C could distinguish the insulin secretion function between NGT group and IGR group (P<0.05). Conclusion The HOMA2-%S-TI calculated from the new homeostasis model is a better index in assessing the insulin sensitivity, and HOMA2-%B-C is a better index in evaluating the insulin secretion function.