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Subcutaneous administration of insulin preventing diabetes is correlated with regulatory T cells and Th cytokine shift in NOD mice
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- Author:
- No author available
- Journal Title:
- CHINESE JOURNAL OF DIABETES
- Issue:
- 12
- DOI:
- 10.3321/j.issn:1006-6187.2007.12.019
- Key Word:
- 小鼠;胰岛素免疫耐受;糖尿病,1型;细胞因子
Abstract： 目的 观察胰岛素(Ins)对非肥胖糖尿病(NOD)小鼠胰岛炎和糖尿病的影响,并探讨其诱导免疫耐受的机制.方法 60只NOD雌鼠随机分为胰岛素(NPH)处理组和PBS对照组,分别于第4、12、20、28周予皮下注射NPH 6U(60μl)及PBS 60μl+不完全弗氏佐剂60μl.于12周龄观察胰岛炎和胰岛β细胞凋亡;测定血清和脾细胞上清IL-4和干扰素(IFN)γ浓度;检测胰岛内和脾细胞IL-4、IFN-γ mRNA的表达;并行联合过继转移实验.结果 Ins组糖尿病发病率和β细胞凋亡率比PBS组低.血清和脾细胞上清IL-4浓度及胰岛内和脾细胞IL-4mRNA表达较PBS组高,而IFN-γ浓度及IFN-γmRNA表达比PBS组低.过继转移实验Ins组DM发病率比PBS组低.结论 Ins能诱导NOD鼠调节T细胞的产生,使全身和胰岛局部T细胞由Th1向Th2转型,抑制β细胞凋亡,从而预防DM.
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