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Inhibition of adiponectin expression in 3T3 - L1 adipocytes by RNA interfering
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- Author:
- No author available
- Journal Title:
- CHINESE JOURNAL OF DIABETES
- Issue:
- 10
- DOI:
- 10.3321/j.issn:1006-6187.2007.10.006
- Key Word:
- RNA 干扰;脂联素;脂肪细胞,3T3-L1
Abstract： 设计合成3对ACRP30编码基因的反向重复序列,分别定向克隆至载体psilencer 1.0的U6启动子下游,构建了相应的shRNAs表达质粒,并证实了这些重组质粒对成熟脂肪细胞中脂联素蛋白的表达有显著的抑制作用.
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- [2]P E, Scherer,S, Williams,M, Fogliano,etc.A novel serum protein similar to C1q, produced exclusively in adipocytes.[J].1995,270.
- [3]Thomas, Tuschl.Expanding small RNA interference.[J].2002,20(5).