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Construction of eukaryotic recombinant vector of human PPARγ2 and establishment of its stable expression cell line
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- Author:
- No author available
- Journal Title:
- CHINESE JOURNAL OF DIABETES
- Issue:
- 7
- DOI:
- 10.3321/j.issn:1006-6187.2007.07.019
- Key Word:
- 过氧化物酶体增殖物激活受体γ2;转染;成纤维细胞;基因表达
Abstract： 目的 构建人过氧化物酶体增殖体激活受体γ2 (human peroxisome proliferator activated receptor γ2,hPPARγ2)真核表达载体并建立稳定表达hPPARγ2的细胞株.方法 用RT-PCR扩增hPPARγ2的全长cDNA并构建出pcDNA3.1(+)/PPARγ2真核表达载体,经核苷酸序列分析证实.真核表达载体pcDNA3.1(+)/ hPPARγ2转染到HESF细胞,G418筛选,获得稳定表达hPPARγ2的细胞株,检测其在HESF细胞中的表达.结果 核苷酸序列测定证实获得hPPARγ2真核表达载体,RT-PCR、间接免疫荧光染色和Western-blot结果显示转染hPPARγ2的细胞株稳定表达hPPARγ2.结论 构建了hPPARγ2真核表达载体,并获得稳定表达hPPARγ2的细胞株,为进一步研究hPPARγ2的功能奠定基础.
- [1]周丽斌,陈名道.脂肪细胞的分化调控[J].2001,(4).
- [2]左祥生,李果,骆天红,等.过氧化物酶体增殖体激活受体γ2表达诱导NIH3T3细胞向脂肪细胞分化[J].2001,(4).
- [3]徐剑,史轶蘩.过氧化物酶体增殖物激活受体γ与肥胖[J].2004,(6).
- [4]Yeldandi V,Gonzalez FJ,Yeldandi AV,etc.Adipocyte-specific gene expression and adipogenic steatosis in the mouse liver due to peroxisome proliferator-activated receptor gamma1 (PPARgamma1) overexpression.[J].2003,278(1).