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Lipid activates TGF-β/Smad signaling pathway in glomeruli of diabetic rats
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- Author:
- No author available
- Journal Title:
- CHINESE JOURNAL OF DIABETES
- Issue:
- 5
- DOI:
- 10.3321/j.issn:1006-6187.2007.05.012
- Key Word:
- 脂质;糖尿病;信号通路
Abstract： 目的 探讨脂质对糖尿病大鼠肾小球TGF-β/Smad信号通路的影响.方法 糖尿病大鼠随机分成普通饲料组,高脂饲料组、高脂饲料加辛伐他汀干预组(辛伐他汀组)和正常对照组.16周后,采用RT-PCR、免疫组化和Western印迹法检测大鼠肾小球转化生长因子β1(TGF-β1)、转化生长因子Ⅱ型受体(TβRⅡ)、Ⅵ型胶原(ColⅥ)mRNA和蛋白和磷酸化Smad2(p-Smad2)表达的变化.结果 与正常对照组比较,普通饲料组、高脂饲料组、辛伐他汀组肾小球TGF-β1、TβRⅡ、ColⅥ mRNA和蛋白及p-Smad2表达均上调,高脂饲料组上调最为明显;与高脂饲料组比较,辛伐他汀组TGF-β1、TβRⅡ、ColⅥ和p-Smad2表达明显下调.结论 脂质通过激活TGF-β/Smad信号通路,加剧糖尿病肾脏病变;辛伐他汀抑制TGF-β/Smad通路的激活,延缓糖尿病肾病的进展.
- [1]Dominguez JH,Tang N,Xu W,etc.Studies of renal injury III: lipid-induced nephropathy in type II diabetes.[J].2000,57(1).
- [2]M, Isono,A, Mogyorósi,D C, Han,etc.Stimulation of TGF-beta type II receptor by high glucose in mouse mesangial cells and in diabetic kidney.[J].2000,278(5).