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Comparison of transplantation of endothelial progenitor cells and mononuclear cells on the treatment of ischemic disorder in the hind limb of diabetic rats
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- Author:
- No author available
- Journal Title:
- CHINESE JOURNAL OF DIABETES
- Issue:
- 4
- DOI:
- 10.3321/j.issn:1006-6187.2007.04.018
- Key Word:
- 内皮祖细胞;单个核细胞;移植;血管病变
Abstract： 目的 比较内皮祖细胞(EPC)和单个核细胞(MNC)移植改善糖尿病大鼠后肢血管病变的疗效.方法 将大鼠EPC和外周血MNC标记后分别植入糖尿病大鼠缺血后肢.对照组仅植入PBS溶液.ELISA法检测VEGF的含量,免疫组化法计数毛细血管数目.结果 EPC和MNC均能在缺血后肢分化为毛细血管.移植后EPC组和MNC组大鼠缺血肢体局部VEGF含量高于对照组,毛细血管数目也高于对照组.但EPC组和MNC组VEGF含量和毛细血管数目无明显差异.结论 移植EPC及含有相当数量EPC的MNC对糖尿病大鼠后肢血管病变的改善作用相似.EPC可能在外周血单个核细胞移植中发挥主要作用.
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