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Effects of rosiglitazone on the expressions of MCP-1 and PAI-1 induced by acute phase serum amyloid protein A in endothelial cells
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- Author:
- No author available
- Journal Title:
- CHINESE JOURNAL OF DIABETES
- Issue:
- 4
- DOI:
- 10.3321/j.issn:1006-6187.2007.04.009
- Key Word:
- 急性时相血清淀粉样蛋白A;罗格列酮;内皮细胞;单核细胞趋化蛋白1;纤溶酶原激活物抑制因子1
Abstract： 目的 观察急性时相血清淀粉样蛋白A(A-SAA)和PPAR-γ激动剂罗格列酮(RSG)对内皮细胞单核细胞趋化蛋白1(MCP-1)和纤溶酶原激活物抑制因子1(PAI-1)基因表达的影响.方法 分别用不同浓度(0.5 μg/ml、5μg/ml)的A-SAA、RSG(10μmol/L)处理ECV304内皮细胞8 h、24 h后抽提总RNA,用半定量RT-PCR技术检测MCP-1、PAI-1 mRNA的表达.结果 A-SAA能显著促进ECV304细胞MCP-1、PAI-1 mRNA的表达,并呈剂量和时效依赖性(P＜0.01).而RSG能部分减低A-SAA对ECV304细胞MCP-1和PAI-1 mRNA表达的促进作用.结论 A-SAA可通过促进内皮细胞MCP-1、PAI-1的表达和分泌,参与动脉粥样硬化的形成;RSG可通过部分减低ASAA对内皮细胞MCP-1和PAI-1表达的促进作用发挥其抗炎、抗AS的作用.
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