The relationship of Met326Ile variant of p85α regulatory subunit of phosphatidylinositol 3-kinase(PI3-K) with type 2 diabetes
- Author:
- No author available
- Journal Title:
- CHINESE JOURNAL OF DIABETES
- Issue:
- 6
- DOI:
- 10.3321/j.issn:1006-6187.2006.06.008
- Key Word:
- 磷脂酰肌醇3-激酶;基因表达;糖尿病,2型;Diabetes mellitus,type 2
Abstract： Objective To investigate the impact of Met326Ile variant of the p85α subunit of PI3-K and its gene expression on type 2 diabetes.Methods The Met326Ile mutation was determined by polymerase chain reaction(PCR) in 240 diabetes inpatients and 150 healthy controls.RT-PCR and spot density measurement were employed to estimate gene expression. Results The allelic frequency of the Ile326 variant was similar in type 2 diabetes and control(22.5% vs 22.4%,P>0.05),and the distribution of genotypes was in Hardy-Weinberg equilibrium.Data analysis showed Met326Ile variant did not impact on FIns,FPG,HOMA index,BMI and gene expression of PI3-K,though the expression of PI3-K gene was lower in type 2 diabetes than in control group.Conclusion The Met326Ile variant of the p85α regulatory subunit of PI3-K is likely to be functionally normal in type 2 diabetes and health people.
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