Rat bone marrow mesenchymal stem cells are induced to differentiate into islet-liking cells in vitro

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Author:
SUN Ji-ping()
JIA Yan-jie()
SONG Jian-hui()
()
()
Journal Title:
CHINESE JOURNAL OF DIABETES
Issue:
Volume 14, Issue 02, 2006
DOI:
Key Word:

Abstract: Objective To induce bone marrow mesenchymal stem cells (MSCs) to differentiate into insulin-secreting cells in vitro. Methods Using a defined culture medium and technique for transdifferentiation, MSCs from adult SD rats were guided into specific insulin-secreting cells. The expressions of islet-specific hormones and proteins, such as insulin, glucagons and somatostatin were detected and analyzed by indirect immunofluorescence cytochemistry staining before and after the induction. And insulin 1, Pdx-1 and Pax-6 were detected by reverse transcription-PCR (RT-PCR). In addition, insulin secretion was examined using radioimmunoassay. Results The differentiated MSCs which expressed nestin were 44.6±7.3% after 5 h induction, 6.18±8.4% after 24 h induction and disappeared at 14 day culture. Meantime, the differentiated cells had positive immunoreactivity to insulin, glucagon and somatostatin,and expressed insulin 1, Pdx-1, Pax-6 mRNA. The differentiated cells were able to produce higher level insulin, and displayed glucose-dependent insulin release in vitro. Conclusion Adult rat MSCs can be induced to differentiate into insulin-secreting cells in vitro.

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