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Impairments of endotoxin-induced CD14 expression and secretions of TNF-α and IL-6 in alveolar macrophage in experimental type 2 diabetic rat

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Author:
No author available
Journal Title:
CHINESE JOURNAL OF DIABETES
Issue:
1
DOI:
10.3321/j.issn:1006-6187.2006.01.022
Key Word:
糖尿病,2型;巨噬细胞,肺泡;CD14;肿瘤坏死因子;白细胞介素6;Interleukin-6

Abstract: Objective To investigate the mechanism for weakened pulmonary local immune defensive function at levels of cell receptor and immunomolecule in type 2 diabetes mellitus(T2DM) rats with acute lung injury induced by lipopolysaccharide(LPS).Methods Wistar rats were fed with the diets rich in highsucrose and highlipid.T2DM model was developed by intraperitoneal injection with streptozotocin 35 mg/kg of body weight 1 month after the diet treatment.Murine model of endotoxemia/ acute lung injury was produced by injection LPS(1 mg/kg of body weight) via tail vein.The expression of CD14 on alveolar macrophage(AM) surface was assayed by immunohistochemistry and then analyzed with computer imaging system.Radioimmunoassay was used to determine the levels of TNF-α and IL-6 in serum and pulmonary homogenates.Results The expression of CD14 on AM surface and the levels of TNF-α and IL-6 in serum in T2DM rats without LPS challenge were significantly higher than those in normal control rats(P<0.05).There was markedly increased average absorbance(A) value of CD14 and the levels of TNF-α and IL-6 in serum and pulmonary homogenates in both normal control rats and T2DM rats after LPS challenge(P<0.01),but the parameters mentioned above in T2DM rats were significantly lower than those in normal control rats after LPS challenge(P<0.01).Conclusions The expression of CD14 on AM surface and the secretion of TNF-α and IL-6 after LPS challenge are severely decreased in T2DM rats.This might be one of important mechanisms for weakened pulmonary local immune defensive function in T2DM.

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