The relationship between T-786 →C mutation in the 5'-flanking region of the endothelial nitric oxide synthase gene and type 2 diabetes with coronary heart disease

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Author:
XIANG Guang-da()
LIU Hong()
HOU Jie()
()
Journal Title:
CHINESE JOURNAL OF DIABETES
Issue:
Volume 14, Issue 01, 2006
DOI:
Key Word:

Abstract: Objective To study the relationship between T-786→C mutation in the 5′-flanking region of the endothelial nitric oxide synthase gene and type 2 diabetes(T2DM) with coronary heart disease(CHD). Methods One hundred and eighty-six T2DM were selected,and of them,65 had CHD.Meanwhile,63 healthy individuals were selected as control.PCR/ASO(allele-specific oligonucleotide probe) technique was used to determine the T-786→C mutation.Results Compared with control,the T/C genotype and C allele prevalences were significantly higher in T2DM with CHD(P<0.05).Compared with T2DM without CHD,the C allele prevalence was significantly higher in T2DM with CHD(P<0.05).Compared with T/T genotype group,the HbA1c level of T2DM was significantly higher in T/C genotype group(P<0.05).By multiple logistic regression analysis,age,blood pressure,duration of diabetes,LDL-C,Lp(a),HbA1c,C allele were associated with CHD development in T2DM(P<0.01).Conclusions T-786→C mutation in the 5′-flanking region of the endothelial nitric oxide synthase gene increases the risk for CHD development in T2DM.

  • [1]Ross GP,Cesari M,Zanchetta M,et al.The T-786C endothelial nitric oxide synthase genotype is a novel risk factor for coronary artery disease in Caucasian patients of the GENICA study.Am Coll Cardiol,2003,41:930-937.
  • [2]Nakayama M,Yasue H,Yoshimura M,et al.T-786→C muta tion in the 5'-flanking region of the endothelial nitric oxide synthase gene is associated with coronary spasm.Circulation,1999,99:2864-2870.
  • [3]Guangda X,Bangshun X,Xiujian L,et al.Apo e4 allele increases the risk for exercise-induced silent myocardial ischemia in non-insulin dependent diabetes mellitus.Atherosclerosis,1999,149:293-296.
  • [4]Nakayama M,Yasue H,Yoshimura M,et al.T-786→C mutation in the 5'-flanking region of the endothelial nitric oxide synthase gene is associated with myocardial infarction,especially without coronary organic stenosis.Am J Cardiol,2000,86:628-634.
  • [5]Asakimori Y,Yorioka N,Taniguchi Y,et al.T-786→C polymorphism of the endothelial nitric oxide synthase gene influences the progression of renal disease.Nephron,2002,91:747-751.
  • [6]Ohtoshi K,Yamasaki Y,Gorogawa S,et al.Association of (-)786T-C mutation of endothelial nitric oxide synthase gene with insulin resistance.Diabetologia,2002,45:1594-1601.
  • [7]Miyamoto Y,Saito Y,Nakayama M,et al.Replication protein A1 reduces transcription of the endothelial nitric oxide synthase gene containing a-786T→C mutation associated with coronary spastic angina.Hum Mol Genet,2000,9:2629-2637.
  • [8]Bonetti PO,Lerman LO,Lerman A.Endothelial dysfunction:A maker of atherosclerotic risk.Arterioscler Thromb Vas Biol,2003,23:168-175.
  • [9]Guangda X,Yuhua W.Apolipoprotein e4 allele and endothelium-dependent arterial dilation in type 2 diabetes mellitus without angiopathy.Diabetologia,2003,46 514-519.
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