Changes in protein kinase B (PKB) expression and glucose transporter 4 (GluT-4) translocation in skeletal muscle of insulin resistant rats

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Author:
BI Hui-min()
OUYANG Jing-ping()
DENG Xiang-qun()
()
()
()
Journal Title:
CHINESE JOURNAL OF DIABETES
Issue:
Volume 13, Issue 04, 2005
DOI:
Key Word:

Abstract: Objective To study the changes in PKB expression and GluT-4 translocation in skeletal muscle of insulin resistant rats fed with high fat diet and the effect of intervention treatments. Methods Normal male SD rats were divided into two groups taking either normal diet(A,n=9) or high-fat diet(B,n=41). After two months, rats in group B were randomly divided into four groups: high-fat diet group(C,n=9), normal diet plus intervention group(D,n=9), kakonein intervention group(E,n=11) and rosiglitazone intervention group(F,n=12). After another one month intervention treatment, their PKB expression and GluT-4 translocation of skeletal muscle were tested. Results Group C developed obvious insulin resistance(IR), and their PKB expression and insulin-stimulated translocation of GluT-4 to plasma membrane in skeletal muscle decreased significantly compared with normal control (group A)(P<0.01). Compared with group C, the PKB expression and insulin-stimulated translocation of GluT-4 to plasma membrane increased obviously in group D,E,F (all P<0.01). Conclusions High-fat fed SD rats can develop obvious IR. Their insulin-stimulated PKB expression and translocation of GluT-4 to plasma membrane in skeletal muscle decrease obviously. Intervention with diet, kakonein or rosiglitazone can increase PKB expression and insulin-stimulated translocation of GluT-4 to plasma membrane in skeletal muscle.

  • [1]Tremblay F, Lavigne C, Jacques H, et al. Dietary cod protein restores insulin-induced activation of phosphatidylinositol 3-kinase/Akt and GluT-4 translocation to the T-tubules in skeletal muscle of high-fat-fed obese rats. Diabetes,2003, 52:29-37.
  • [2]陈小燕,周智广,唐炜立,等.多因素干预对新诊断2型糖尿病患者代谢综合征的影响.中华糖尿病杂志,2004,12:173-176.
  • [3]Cho H, Mu J, Kim JK,et al. Insulin resistance and a diabetes mellitus-like syndrome in mice lacking the protein kinase Akt2 (PKB beta). Science,2001,292:1728-1731.
  • [4]于健,苏柯. 葛根素对2型糖尿病病人胰岛素抵抗的影响.中国新药与临床杂志, 2002, 21:585-587.
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