Abstract： Objective To study the changes in PKB expression and GluT-4 translocation in skeletal muscle of insulin resistant rats fed with high fat diet and the effect of intervention treatments. Methods Normal male SD rats were divided into two groups taking either normal diet(A,n=9) or high-fat diet(B,n=41). After two months, rats in group B were randomly divided into four groups: high-fat diet group(C,n=9), normal diet plus intervention group(D,n=9), kakonein intervention group(E,n=11) and rosiglitazone intervention group(F,n=12). After another one month intervention treatment, their PKB expression and GluT-4 translocation of skeletal muscle were tested. Results Group C developed obvious insulin resistance(IR), and their PKB expression and insulin-stimulated translocation of GluT-4 to plasma membrane in skeletal muscle decreased significantly compared with normal control (group A)(P<0.01). Compared with group C, the PKB expression and insulin-stimulated translocation of GluT-4 to plasma membrane increased obviously in group D,E,F (all P<0.01). Conclusions High-fat fed SD rats can develop obvious IR. Their insulin-stimulated PKB expression and translocation of GluT-4 to plasma membrane in skeletal muscle decrease obviously. Intervention with diet, kakonein or rosiglitazone can increase PKB expression and insulin-stimulated translocation of GluT-4 to plasma membrane in skeletal muscle.