Abstract： ObjectiveTo establish hyperglycemic clamp tech ni que, a method for the precise measurement of insulin secretion.Methods Hyperglycemic clamp research were conducted on 25 normal glucose toler ance Chinese individuals from Shanghai, 16 men, and 9 women. The subjects were f asting. Intra-catheters were placed in both upper limbs veins. One side was for infusion of 20% glucose solution continually to keep plasma glucose 7.9 mmol/L higher than basic glucose state during the test by negative feedback principle. The other side was for the collection of blood samples to obtain insulin value a nd two-phases insulin secretion so as to evaluate β cell function.Re sults(1)The plasma glucose concentration of the individuals reached a hyperglycemic plateau in prescriptive time (14 minutes)(12.86±0.11 mmol/L)a nd kept the level during the whole test. (2)Throughout the hyperglycemic clamp process, the variance coefficient of plasma glucose which was determined every 5 minutes was 4.2%. (3)It was observed that two-phases of insulin secretions were present during persistent hyperglycemic state with phase 1 of (247.8±23 .5) mU/L, and phase 2 of (72.8±6.1) mU/L.(4)It was obtained that insulin sens itivity index (19.93±2.43) in hyperglycemic clamp was higher than that in eugl ycemic clamp.Conclusions(1)Hyperglycemic clamp technique i s established successfully . It may evaluate the two-phases insulin release and maximum insulin secretion in normal individuals. (2) Hyperglycemic clamp can also evaluate insulin resistance and observe the discrepancy of insulin sensitiv ity in different populations simultaneously.