Interferon-γ up-regulates interleukin-18 and caspase-1 expression in islet β-cells
- Author:
- No author available
- Journal Title:
- CHINESE JOURNAL OF DIABETES
- Issue:
- 4
- DOI:
- 10.3321/j.issn:1006-6187.2002.04.004
- Key Word:
- 干扰素γ;白细胞介素18;白细胞介素1β转换酶;Interleukin 1β converting enzyme
Abstract： Objective To investigate whether interleukin 18 (IL 18) and IL 1β converting enzyme (ICE, caspase 1) is expressed in islet β cells and the mechanism of their expression control. Methods Rat insulinoma (RIN) cells, FACS purified rat β cells, isolated rat islets, and islets isolated from interferon regulatory factor 1 (IRF 1) gene knockout mice were incubated with or without cytokines. Reverse transcription polymerase chain reaction (RT PCR) was used to quantitate IL 18 and caspase 1 mRNA expression. IL 18 protein expression was detected by using Western blot. Results (1)Interferon γ (IFN γ) increased IL 18 mRNA expression in RIN cells, purified rat β cells, and intact rat islets, and the effect was unchanged in IRF 1 knockout mouse islets. (2) IFN γ highly up regulated caspase 1 mRNA expression in RIN cells and intact rat islets, but the expression was abolished in IRF 1 knockout mouse islets. (3) IL 18 protein was undetectable in lysates and supernatants of RIN cells. Conclusion IFN γ up regulates IL 18 and caspase 1 expression in islet β cells. IL 18 expression is not dependent on a transcription factor IRF 1, while caspase 1 expression is in an IRF 1 dependent way.
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