The experimental study about endostatin inhibiting corneal neovascularization and effects on the expression of flk-1
- Author:
- No author available
- Journal Title:
- Chinese Journal of Practical Ophthalmology（CJPO）
- Issue:
- 9
- DOI:
- 10.3760/cma.j.issn.1006-4443.2012.09.026
- Key Word:
- 内皮抑素;Flk-1;CNV;碱烧伤;ES;Flk-1;Corneal neovascularization;Alkali-burning
Abstract： Objective To investigate the effect of ES on inhibiting CNV and the expression of Flk-1. Method ES and normal saline were separately injected into subconjunctival tissues immediately after alkali-burning,and the manipulation was repeated every other day.The expression of Flk-1 was examined by immunohistochemical method.The length and growth velocity of the newly formed comeal vessels were also measured. Results 1.Flk-1 was not expressed in the cornea.When the cornea was bumed by alkali,the expression was dramatically increased.There was a significant difference between group B and group A (P ＜0.01).2.The expression of Flk-1 was significantly inhibited by ES.There was a significant difference between the group C and group B (P ＜0.01).3.The CNV in the group C formed later and grew more slowly than that of group B (P ＜0.01).Conclusions 1.After alkali-burning,the expression of Flk-1 is greatly enhanced.The increase of Flk-1 is the very important causation of the CNV.2.ES can significantly inhibit the expression of Flk-1,ES can greatly decrease the bioactivity of VEGF by inhibiting the expression of Flk-1.So it can significantly inhibit the forming of CNV.
- [1]苏凡凡,张明昌.姜黄素对大鼠碱烧伤角膜新生血管的抑制作用[J].2008,(9).
- [2]倪庆桂,汪振诚,徐根兴,等.人内皮抑素基因的克隆及其表达产物抗肿瘤活性[J].2002,(1).
- [3]周金子,夏晓波,熊思齐,等.VEGF siRNA对兔眼碱烧伤后角膜VEGF表达的作用[J].2008,(4).
- [4]Wu LW,Dunbar JD,Jaffe EA,etc.Utilization of distinct signaling pathways by receptors for vascular endothelial cell growth factor and other mitogens in the induction of endothelial cell proliferation.[J].2000,275(7).
- [5]Li Y,Xu HZ,Jiang J,etc.Suppression of retinal neovascularization by shRNA targeting HIF-1alpha.[J].2008,33(10).
- [6]Blezinger, Paul,Wang, Jijun,Gondo, Margaret,etc.Systemic inhibition of tumor growth and tumor metastases by intramuscular administration of the endostatin gene.[J].1999,17(4).
- [7]Nobiling R,Kruse FE,Azzam L,etc.In vivo significance of ICAM-1--dependent leukocyte adhesion in early corneal angiogenesis.[J].1999,40(3).
- [8]Standker L.,Kanse SM.,Jurgens M.,etc.Isolation and Characterization of the Circulating Form of Human Endostatin[J].1997,420(2/3).
- [9]N S, Taichman,S, Young,A T, Cruchley,etc.Human neutrophils secrete vascular endothelial growth factor.[J].1997,62.
- [10]P, van der Geer,T, Hunter,R A, Lindberg.Receptor protein-tyrosine kinases and their signal transduction pathways.[J].1994,10.
- [11]Philipp W,Speicher L,Humpel C.Expression of vascular endothelial growth factor and its receptors in inflamed and vascularized human corneas.[J].2000,41(9).
- [12]Gluzman Poltorak-Z,Cohen T,Neufeld G,etc.Neuropilin-2 and neuropilin-1 are receptors for the 165-amino acid form of vascular endothelial growth factor (VEGF) and of placenta growth factor-2, but only neuropilin-2 functions as a receptor for the 145-amino acid form of VEGF.[J].2000,275(24).
- [13]Michael S O'Reilly,Thomas Boehm,Yuen Shing,etc.Endostatin: An Endogenous Inhibitor of Angiogenesis and Tumor Growth[J].1997,88(2).