Objective To investigate the clinicopathology characteristics of asopharyngeal diffuse large B-cell lymphoma(DLBCL) and improve diagnosis of the disease. Methods The clinical, immunophenotypic and histopathologic features of 12 cases asopharyngeal DLBCL were retrospectively studied. The expression of CD20, CD3, CD10, BCL6, MUM-1, Ki-67, CD30, AE1/3, CD30, ALK, CD5and CyclinD1 was detected. Results All patients were primary DLBCL. Among the 12 patients,8 were males and 4 were femals. The age ranged from 34 to 67 years, average age 48 years old. Among them,7 cases were germinal centre B-cell-like(GCB) , 5cases were non-germinal centre B-cell-like(non-GCB). Conclusion Asopharyngeal DLBCLs are easily misdiagnosed as carcinomas. The correct diagnosis of DLBCL may be reached by combination of clinic, histological and immunological features.