Objective To observe ganoderma lucidum spore powder perishes weakly on type 2 diabetes big mouse testis cell IL-1 β、TNF-α expression influence. Methods 50 Wistar big mouse randomly were divided into 3 groups control group, diabetes group, ganoderma lucidum group. Radioimmunoassay examines testis cell factor IL-1β、TNF-α levels. Results Diabetes group and the normal control group, IL-1β and TNF-α were significantly higher(P＜ 0. 05). The ganoderma lucidum group and diabetes group, IL-1β、TNF-α levels significantly lower than diabetes group (P＜ 0.05). Conclusion The ganoderma lucidum spore can effectively reduce the IL-1 β, TNF-α levels in diabetes rats toxic damage to the reproductive system, probably in diabetes rats reproductive system of apoptosis significantly inhibition of testicular cells so protective effect from one of the mechanisms. Ganoderma lucidum spore as a complementary treatment of diabetes treatment in clinical applications,has broad prospects.