Abstract： Objective The reticulocytes in different conditions and its related parameters were evaluted by the XE-2100 automated hematology analyzer, so we can understand the precision of instrumental analysis and the reference range of healthy adults, but also the reticulocyte change in the anemia condition. Methods We selected ten normal human fresh blood samples to nake replicate test, and invested each parameters average of 200 healthy adults and 116 anemia specimens in order to study the change of the HLR and IRF with different causes. Resuits The Cv of the RET replicate test is 3.4% , and the RET range of normal humen being is between 0. 58% and 1.88%. The normal HLR mean is 0. 32% ;but each HLR value of the anemia with different causes including hemolytic anemia, acute hemorrhage, chronic anemia、leukemia and aplastic anemia is 4. 13 % ,1.73% ,0. 76% ,0. 34% ,0. 30% separately. The change of the MSCV value is unnormal ,and it is about 23% in anemia specimens, but 7.2% in healthy samples. Conclusion The precision and accuracy of the instrumental analysis is.