Abstract： Objective To investigate the prognostic value of CD44V6,MMP-9 and HCG in mallgnant in transformation of hydatidiform mole.Methods The expressions of CD44V6 and MMP-9 was detected by S-P method and the serum HCG was detected with CLIA method in 53 cases of hydatidiform mole and the relationship of the expression was analyzed.Results There were 9 cases malignant transforming hydatidiform mole and 44 eases of non-malignant transforming hydatidiform mole.The expression of CD44V6 and MMP-9 of malignant transforming mole(77.8% and 66.7%)significantly higher than that of non-malignant transforming group(31.8%and 43.2%) (P ＜ 0.05).Conclusion CD44v6 and MMP-9 play important roles in the process of transformation of hydatidiform mole.The combined detection of CD44V6,MMP-9 and HCG can help to prodict biological behaviors and prognosis of hydatidiform mole.