Abstract： Objective To comparative investigate the influence of AA-861 alone or in combination with Celecoxib on the proliferation of colon carcinoma HT-29 cell line.Methods Group cultured colon cancer HT-29 cells ,The morphological changes of HT-29 cells was observed by inverted phase contrast microscope after Docebenone(AA-861)and Celecoxib alone or in combination;By cell proliferation assay (MTT)detect cell absorbance so as to test cell proliferation;The expression change of VEGF was detected by Immunofluorescence staining experiments under Confocal microscope.Results We can observe that cell morphology chaned significantly under the inverted phase contrast microscope,and the group of AA-861and Celecoxib in combination changed most obvious; These results of MTT suggest that the group of two drugs in combination shows the lowest rate of cell proliferation.Immunofluorescence staining experiments revealed that the expression of VEGF was decreased,and the group of two drugs in combination has the lowest expression.Conclusion AA-861 and Celecoxib in combination can produce a superior anti-colon cancer effection than AA-861 or Celecoxib alone,and this effect may be related with inhibit the expression of VEGF.