You Position: Home > Paper
Effect of insulin on endothelial cell apoptosis and expression of bax and bcl-2 gene in hyperglycemia
( views:216, downloads:0 )
- Author:
- No author available
- Journal Title:
- CHINA CLINICAL PRACTICAL MEDICINE
- Issue:
- 8
- DOI:
- No doi available
- Key Word:
- 胰岛素;葡萄糖;内皮细胞;细胞凋亡
Abstract： 目的 为探讨不同浓度胰岛素对高浓度葡萄糖培养的人脐静脉内皮细胞的凋亡及对凋亡基因bax和bcl-2表达的影响.方法 人脐静脉内皮细胞ECV304在不同浓度胰岛素(10 U/L、20 U/L、40 U/L)和高葡萄糖浓度(20 mmol/L)培养48 h后,噻唑蓝(MTT)方法检测细胞存活率,PT-PCR检测bax和bcl-2的mRNA表达.结果 高糖能诱发内皮细胞凋亡,在胰岛素浓度为10 U/L环境下,细胞的生存率明显高于单纯高糖组,但随着胰岛素浓度的升高,细胞的生存率下降;在胰岛素浓度为10 U/L时,bax mRNA显著减少,bcl-2显著增加;当胰岛素浓度升高至40 U/L时则相反.结论 适当浓度的胰岛素能保护高糖环境下的内皮细胞,其机制可能与bcl-2基因表达上调有关.
- [1]K L, Chien,Y T, Lee,F C, Sung,etc.Hyperinsulinemia and related atherosclerotic risk factors in the population at cardiovascular risk: a community-based study.[J].1999,45.
- [2]X. L. Du,G. Z. Sui,K. Stockklauser-Färber,etc.Induction of apoptosis by high proinsulin and glucose in cultured human umbilical vein endothelial cells is mediated by reactive oxygen species[J].1998,41(3).
- [3]Choy JC,Hunt DW,McManus BM,etc.Endothelial cell apoptosis: biochemical characteristics and potential implications for atherosclerosis.[J].2001,33(9).