Abstract： Objective To investigate the expression of AP-1 in pancreatic tumor cells and its clinical significance.Methods Selected the paraffin-embedded specimens from the tumor tissues of 35 patients with pancreatic cancer(study group) and 35 pancreatic tissue of healthy people(control group),and double antibody sandwich ABCELISA detection of AP-1 was carried out,two sets of specimens C-JUN and C-FOS expression was observed.Results Observation group C-JUN and C-FOS expression positive rates were 80.00％ 71.43％,control group,C-JUN and C-FOS expression positive rates were 22.86％,28.57％,there was a significant difference between the two groups.Conclusion AP-1 was highly expressed in pancreatic cancer,which could guide clinical take reasonable interventions to slow tumor development.