Effent of toosendanin on the biological behavior of human hepatoma cell line HepG2

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Author:
ZHAO Fei(Department of the Traditional Chinese Medicine, Qianfoshan Hospital ,Jinan , Shandong 250014, China)
Journal Title:
Chinese Journal of Primary Medicine and Pharmacy
Issue:
Volume 19, Issue 20, 2012
DOI:
10.3760/cma.j.issn.1008-6706.2012.20.003
Key Word:
Toosendanin; HepG2 ; Biological markers

Abstract: Objective To observe the effect of toosendanin on proliferation and apoptosis of human hepatoma cell line HepG2 cells,and to study the mechanism.Methods Human hepatocellular carcinoma HepG2 cells were randomly divided into control group(group A) does not add any medication,the observation group(group B) joined TSN 80mmol/ml,cultured cells for 24,48,72 hours.HepG2 cells proliferation inhibition rate was measured by MTT; Hepatocellular carcinoma HepG2 cell cycle and apoptosis rate was deteded by flow cytometry.Results TECAN determination revealed two groups of hepatocellular carcinoma cell proliferation had statictical significance ( x2 =5.33,P <0.05 ) ;flow cytometry revealed two groups S,G0/G1,M/G2 phase of the cell rate ( t =6.31,6.26,6.56,all P <0.05 ),apoptosis rate had significant difference ( x2 =6.15,P < 0.05 ).Conclusion Toosendanin could obviously inhibit the proliferation of HepG2 cells,and induce apoptosis of tumor cells.

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