Sorting of side population cells from breast cancer MCF-7 cell line and its biological characteristics
- Author:
- No author available
- Journal Title:
- Chinese Journal of Primary Medicine and Pharmacy
- Issue:
- 13
- DOI:
- 10.3760/cma.j.issn.1008-6706.2012.13.004
- Key Word:
- MCF-7乳腺癌细胞系;侧群细胞;增殖;MCF-7 breast cancer stem cell;Side population cell;Proliferation
Abstract： Objective To separate the side population cells(SP) from breast cancer MCF-7 cell line,and observe its biological characteristics.Methods Flow cytometry and Hcechst 33342 dye efflux assay were used to isolate SP cells and non-SP cells from the MCF-7 cell line of human breast cancer.Tumorigenicity of the two subpopulations was observed by a soft agar cloning method.Results The results of FACS analysis indicated that (6.5 ± 0.4 ) ％of the MCF-7 cells were SP cells;The vitro colony formation rate of SP cells was(38.5 ±9.4)％,and higher than that of non-SP cells ( 8.4 ± 2.6 ) ％ ( t =5.34,P ＜ 0,05 ).Concluslon The SP cells sorted from MCF-7 cell line enriched tunor stem cells,which exhibited high tumorigenicity.It indicated that SP cells should play a principal role in breast cancer.
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