Isolation of intraperitoneal free cancer cells from colorectal cancer by immunomagnetic beads
- Author:
- No author available
- Journal Title:
- Chinese Journal of Primary Medicine and Pharmacy
- Issue:
- 6
- DOI:
- 10.3760/cma.j.issn.1008-6706.2012.06.002
- Key Word:
- 结直肠肿瘤;免疫磁珠;细胞学技术;Colorectal cancer;Immunomagnetic beads;Cytological techniques
Abstract： Objective To explore the value of immuomagnetic beads(IMB) technique for detection of intraperitoneal free cancer cells from colorectal cancer.Methods Peritoneal lavage fluid was obtained from 80 patients with colorectal cancer during laparotomy.Peritoneal lavage cytology (PLC) and IMB were used to detect free cancer cells in peritoneal lavage fluid.10 patients with hysteromyoma during laparotomy were enrolled into the control group.Results The positive rate of PLC was 8.8％ (7/80),the positive rate of IMB was 28.8％ (23/80).The positive case after useing PLC detect,IMB detect also was positive.The detected samples of control group were negative by these two methods.IMB was superior to PLC ( x2 =10.503,P =0.001 ).Conclusion IMB was more sensitive and specific than PLC,which could provide a effective method for finding intraperitoneal free cancer cells.
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