Intestinal lymph drainage reduces lung tissue cell apoptosis of hemorrhagic shock rats
- Author:
- No author available
- Journal Title:
- Chinese Journal of Primary Medicine and Pharmacy
- Issue:
- 6
- DOI:
- 10.3760/cma.j.issn.1008-6706.2012.06.001
- Key Word:
- 休克,出血性;肺损伤,急性;肠系膜淋巴管;细胞凋亡;Shock,hemorrhagic;Lung injury,acute;Mesenteric lymph duct;Apoptosis
Abstract： Objective To explore the effect of the intestinal lymph drainage on lung tissue cell apoptosis in rats with hemorrhagic shock after resuscitation,rich ALI intestinal lymphatic pathway theory.Methods TUNEL method was used to determine the apoptosis of lung tissue cells,the brown nuclei were apoptotic cells.SABC was used to determine Bcl-2 and Bax protein expression.Results The shock group and shock + drainage group lung tissue cell apoptosis rate were significantly higher than those in the sham operation group,but the shock + drainage group,the apoptosis of lung tissue cells was significantly lower than the shock group.Sham operation group showed Bcl-2,Bax protein of expression; In shock group,lung tissue cell Bcl-2 expression was significantly lower than the sham operation group,the Bax expression was significantly higher than that in the sham operation group; In shock + drainage group,lung tissue cells shored enhanced expression of Bcl-2,Bax expression was reduced,and the shock + drainage group lung tissue cell Bcl-2 expression was significantly higher than that in the shock group,the expression of Bax was significantly lower than the shock group.Conclusion The excessive apoptosis of lung tissue cells was one of the mechanisms of lung injury after shock.Intestinal lymph drainage could reduce lung tissue cell apoptosis,the mechanism invdved the regulation of Bcl-2/Bax protein expression.
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