You Position: Home > Paper

Expression of GDNF and ChAT in the hippocampus of STZ-induced diabetic rats and relation between GD-NF and cognitive function

( views:186, downloads:24 )
Author:
No author available
Journal Title:
Chinese Journal of Primary Medicine and Pharmacy
Issue:
24
DOI:
10.3760/cma.j.issn.1008-6706.2011.24.006
Key Word:
糖尿病;认知功能障碍;胶质细胞源性神经营养因子;胆碱乙酰转移酶;逆转录,聚合酶链式反应;Diabetes mellitus;Cognitive dysfunction;Glial cell line-derived neurotrophic factor;Choline acetyltransferase;Reverse transcription,polymerase chain reaction

Abstract: Objective To study the relationship between GDNF and ChAT Mrna and their potential role in the diabetic cognitive dysfunction by observing the change of the expression of GDNF and ChAT mRNA in brain under different blood glucose levels in STZ induced diabetic rats.Methods Forty male Wistar rats were randomly divided into diabetic group without blood glucose control( DM1 group),diabetic rats treated with insulin group( DM2 group),and normal control group( NC group).Blood glucose and body weight were detected every 4 weeks.Twelve weeks later,the hippocampus were submitted for cryostat sections which were subjected to the reverse transcription-polymerase chain reaction(RT-PCR) for GDNF and ChAT mRNA.Results The expression of GDNF mRNA and ChAT mRNA in the hippocampus have negative correlation with HbAlc level( r =-0.962,-0.974,all P < 0.001 );The GDNF expression had positive correlation with the ChAT mRNA expression( r =0.974,P < 0.001 ).Conclusion Longterm chronic hyperglycation caused the “downregulation” of the GDNF expression in the hippocampal,which could cause the central cholinergic dysfunction symbled with the decrease of ChAT mRNA expression and the decline of learning and cognition ability.

WanfangData CO.,Ltd All Rights Reserved
About WanfangData | Contact US
Healthcare Department, Fuxing Road NO.15, Haidian District Beijing, 100038 P.R.China
Tel:+86-010-58882616 Fax:+86-010-58882615 Email:yiyao@wanfangdata.com.cn