Expression and clinical significance of TRAIL and Caspase-3 in the BMMNC of aplastic anemia

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Author:
CHEN Jin-wen(Department of Clinical Laboratory,Zhuhai Hospital of Guangdong Provincial Traditional Chinese Medicine Hospital, Zhuhai , Guangdong 519015, China)
GUO Guo-wei(Department of Clinical Laboratory,Zhuhai Hospital of Guangdong Provincial Traditional Chinese Medicine Hospital, Zhuhai , Guangdong 519015, China)
CHEN Ren-an()
Journal Title:
Chinese Journal of Primary Medicine and Pharmacy
Issue:
Volume 18, Issue 17, 2011
DOI:
10.3760/cma.j.issn.1008-6706.2011.17.005
Key Word:
Anemia aplastic;TNF related apoptosis-inducing ligand;Caspase-3

Abstract: Objective To study the expression and clinical significance of TNF related apoptosis-inducing ligand(TRAIL) and Caspase-3 in the bone marrow mononuclear cell(BMMNC) of aplastic anemia(AA) ,and to approach their effect on the apoptosis of haemopoietic stem cell in AA patients.Methods The TRAIL and Caspase-3 expression in 15 cases with newly diagnosed AA patients and remission patients,and 15 cases with normal bone marrow patients were detected by flow cytometry and microplate reader. Results The TRAIL expression of newly diagnosed AA patient, emission AA patients and control group was ( 5.94 ± 2. 57 ) %, ( 2. 87 ± 1. 72 ) % and ( 3.01 ±2. 06)% respectively,the Caspase-3 expression of newly diagnosed AA patient, emission AA patients and control group was ( 1. 3840 ± 0. 0236), (0. 8018 ± 0. 0126) and ( 0. 7441 ± 0. 0112 ) respectively. The TRAIL and Caspase-3 expression of newly diagnosed AA patient were significantly higher than the remission AA patients and control group,the difference was significant( all P <0. 05) ;The TRAIL and Caspase-3 expression of control group were higher than the remission AA patients, but the difference was not significant ( all P > 0. 05 ). Conclusion The TRAIL and Caspase-3 had high expression in the BMMNC of AA patients, and the apoptosis induced by TRAIL and Caspase-3 should play an important role in the pathogenesis of AA.

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