Experimental analysis the first cases of cholera epidemic detection of 0139 of Huainan

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Author:
YU Shou-Jie(Huaonan municipal cecter for Disease Control and Prevention,Huainan,Anhui 232000, China)
LIU Xiao-mei(Huaonan municipal cecter for Disease Control and Prevention,Huainan,Anhui 232000, China)
CAO Min(Huaonan municipal cecter for Disease Control and Prevention,Huainan,Anhui 232000, China)
HU Shou-kui()
HU Wan-fu()
Journal Title:
Chinese Journal of Primary Medicine and Pharmacy
Issue:
Volume 18, Issue 16, 2011
DOI:
10.3760/cma.j.issn.1008-6706.2011.16.003
Key Word:
Cholera;Cholera toxin;Toxin coregulated pilus;Turtle

Abstract: Objective To identify the first cases of cholera in Huainan,0139 epidemic causes and biological properties. Methods Conventional methods of serological and bacteriological methods of the strains were identified by polymerase chain reaction (PCR) to detect cholera virulence genes, susceptibility testing modified KB.Results 1 ,was isolated and serum agglutination results,2 patients were all positive stool samples,6 were in close contact with all the negative rectal swab;turtle pond-like 18,5 were positive;turtle egg in battle were like 9,2 were positive ; turtle eggs 7, the positive three copies; turtle waste 11, were negative. 2, or more positive culture drug sensitivity test of the pioneer B, doxycycline resistant; to ampicillin, tetracycline in sensitive;on norfloxacin, gentamicin, ciprofloxacin sensitive. 3,or more positive cultures gene DNA PCR test results,cholera toxin(CT) ,toxin coregulated pilus (TCP) all were positive. Conclusion From the outbreak of cholera 0139 is water pollution caused by turtle ponds,The trip types of 0139 Vibrio cholera were highly homologous with that isolated from green turtle;the bacteria of the pioneer B, doxycycline resistance and carrying cta, tcpa virulence genes.

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