Detecting KRAS,EGFR mutations in nonsmall-cell lung cancer/colorectal cancer patients by high resolution melting curve analysis
- Author:
- No author available
- Journal Title:
- CHINESE JOURNAL OF PRIMARY MEDICINE AND PHARMACY
- Issue:
- 4
- DOI:
- 10.3760/cma.j.issn.1008-6706.2011.04.003
- Key Word:
- 非小细胞肺癌;结直肠癌;突变;KRAS基因;EGFR基因;高分辨率熔解曲线;Nonsmall-cell lung cancer;Colorectal cancer;Mutation;KRAS gene;EGFR gene;High resolutionmelting curve analysis
Abstract： Objective To evaluate the feasibility of detecting the mutations of KRAS,EGFR in nonsmall-cell lung cancer and colorectal cancer patients with high resolution melting curve analysis. Methods At first,the mutations in KRAS exon 2 and EGFR exons 18 to 21 of 5 patients with non-small cell lung cancer and 5 patients with colorectal cancer were detected by high resolution melting curve analysis.Then the results were confirmed with direct sequencing. Results By high resolution melting curve analysis,1 of 10 patients was found to carry EGFR 19 mutation who was harboring 2236-2250del mutation.By direct sequencing,consistent results of the patients with mutations orwithout mutations were got. Conclusion s The new high resolution melting curve analysis was more efficient,more convenient than direct sequencing.Moreover,it was a low-cost test,which was suitable for clinic test.
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