Effects of dexamethasone on expressions of epithelial neutrophil activating protein-78 and transforming growth factor-beta 1 in neutrophil of asthma rats

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WANG En-zhi()
LUO Dong-jiao()
TONG Xia-sheng(Department of Pediatrics, the Integrated Traditional and Western Medicine Hospital of Taizhou , Taizhou ,Zhejiang 317523, China)
ZHOU Xinhui()
YE Hui()
GUO Hai-yuan()
RUAN Zheng-ying()
JIN Xiao-hong()
Journal Title:
Volume 17, Issue 22, 2010
Key Word:
Asthma;Epithelial neutrophii activating protein-78;Transforming growth factor betal;Neutrophil;Dexamethasone

Abstract: Objective To investigate the effects of dexamethasone on expressions of epithelial neutrophil activating protein-78 (ENA-78) and transforming growth factor- beta 1 (TGF-β1) in neutrophil of asthma rats.Methods Thirty male SD rats were randomly divided into three groups on average, including asthma group, control group and dexamethasone treated group. In this experiment, the rat model of asthma were established by sentization and challenge with ovalbumin. Blood neutrophil were isolated and purified. The expression of ENA-78 was detected by flow cytometry. The expression of TGF-β1 was detected by immunohistochemical method in blood neutrophil and bronchial wall. Results Expression of ENA-78 in blood neutrophil in dexamethasone treated group(71.82 ±8. 87 mean fluorescence intensity)was lower than that in asthma group, but higher than that in control group(all P <0. 01). And expressions of TGF-β1 protein in dexamethasone treated group(0. 173 ± 0. 014,0. 202 ± 0. 019 optical density, respectively) was lower than that in asthma group(all P <0. 01) ,but higher than that in control group(all P <0. 01). There were significant positive correlation between ENA-78 expression at blood neutrophil and numbers of total inflammation cells in bronchoalveolar lavage fluid (n = 29, γ = 0. 762, P < 0. 01). Conclusion The beneficial effect of glucocorticoid(dexamethasone) on airway inflammation in asthma rats could be at least in part due to their direct inhibitory effect on ENA-78 and TGF-β1 protein generation by neutrophil.

  • [1]林剑.中性粒细胞在支气管哮喘发病机制中的作用.国外医学儿科学分册,2001,28(1):22-24.
  • [2]童夏生,李昌崇.中性粒细胞分泌功能与支气管哮喘.国际呼吸杂志,2006,26(1):45-48.
  • [3]童夏生,李昌崇,李绍波.中性粒细胞及其CD11b在大鼠哮喘中的表达及地塞米松调控.温州医学院学报,2005,35(3):207-210.
  • [4]郑吉善,苏苗赏,李昌崇,等.地塞米松对变应性哮喘小鼠胸腺活化调节趋化因子及mRNA表达的影响.中国药学杂志,2007,42(22):1709-1713.
  • [5]李文,陈敏,黎东明,等.STAT6基因多态性与哮喘的相关性对血浆IgE水平的影响.中国基层医药,2007,14(5):764-766.
  • [6]Message SD,Johnston SL.Host defense function of the airway epithelium in health and disease:clinical background.J Leukoc Biol,2004,75(1):5-17.
  • [7]刘传合,陈虹,陈育智.趋化因子及其受体在支气管哮喘中的作用.国外医学儿科学分册,2003,30(5):231-233.
  • [8]Suzumori N,Katano K,Suzumori K.Peritoneal fluid concentrations of epithelial neutrophil-activating peptide-78 correlate with the severity of endometriosis.Fertil Steril,2004,81 (2):305-308.
  • [9]徐毛冶,黄茂.转化生长因子β1与支气管哮喘的关系.国外医学呼吸系统分册,2005,25(4):275-276.
  • [10]Goulet S,Bihl MP,Gambazzi F,et al.Opposite effect of corticosteroids and long-acting beta2 agonists on serum and TGF-betalinduced extracellular matrix deposition by primary human lung fibroblasts.J Cell Physiol,2007,210(1):167-176.
  • [11]侯芸.调节性T细胞、TGF-β与系统性红斑狼疮.中国中西医结合皮肤性病学杂志,2005,4(2):127-130.
  • [12]Torrego A,Hew M,Oates T,et al.Expression and activation of TGF-beta isoforms in acute allergen induced remodelling in asthma.Thorax,2007,62(4):307-313.
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