Abstract： Objective To investigate the effect of the UU and CT in the infertility.Methods Tractus genitalis secretion of 305 infecund women and 130 normal women were tested by realtime PCR and the UU and CT were analyzed.Results The infection rat of UU,CT and UU + CT in the test group were 59.3% 、41.0% and 17.0% which were all higher than that of control group(χ2 = 89.82、44.86、19.11,all P＜0.05).However,the difference couldn't be seen between the primary infertility group and the secondary infertility group.Conclusion The women's infertility was related to the infection of the UU and CT.